Learn how to use this product
biotechrabbit™ YourTaq Hot Start DNA Polymerase is optimized for a high amplification yield of 0.1–3 kb DNA targets, even from low copy number. The enzyme shows excellent PCR specificity and sensitivity for various amplicons. The enzyme is resistant to PCR inhibitor carry-over, such as blood (up to 20%), Ethanol, or humic acid, enabling PCR amplification from DNA templates with carry-over of PCR inhibitors.
YourTaq Hot Start DNA Polymerase is a highly pure enzyme. Together with the optimized buffer and high-quality dNTPs (BR BR0600202), a mix is achieved for the most demanding PCR applications. In addition, the enzyme is suitable for the amplification of GC-rich templates (up to 70%) when paired with the 5X PCR Enhancer (BR BR1900201).
Info: The recommended annealing temperature is 2°C above primer Tm (use gradient PCR to optimize the annealing temperature).