Simple, rapid, and efficient electrophoretic tissue clearing
Spend your time more efficiently and decrease your time spent
on preparing, sectioning, labeling, mounting, imaging, and analyzing those hundreds of ultra-thin slices when you want to analyze tissues.
The X-CLARITY II offers you the ultimate solution for electrophoretic tissue clearing: prepare your tissue in its whole for further
histology and imaging analysis.
Tissue clearing with X-CLARITY II: how it works With X-CLARITY II you clear whole tissues from their lipid bilayers (through
electrophoresis in the presence of ionic detergents) while keeping the biomolecules intact, which are covalently linked to a sturdy
hydrogel network. This tissue-clearing method preserves the molecular information and structural integrity of a tissue sample and leaves
you with a stable and transparent tissue-hydrogel hybrid. This hybrid can be efficiently penetrated by and labeled with antibodies or
oligonucleotides for further phenotyping.
Experience the upgraded X-CLARITY Tissue Clearing II with enhanced features: - Innovative &
simple touchscreen interface New & improved module for precise temperature control - Active and passive clearing modes
Exportable data monitoring clearing conditions - Clearing chamber with plated electrodes for steady electric field
Due to the active and passive tissue clearing modes and the availability of holders of various sizes, a wide range of tissue types can
be cleared with the X-CLARITY II. See more tissues that can be cleared with the X-Clarity here. Tissue clearing workflow
The superfast and efficient workflow of X-CLARITY II tissue clearing involves four steps:
Incubation of the tissue sample in a hydrogel monomer solution to allow uniform hydrogel diffusion throughout the whole sample
Polymerization to form the stable hydrogel-tissue hybrid Clearing of the lipid bilayers in the tissue-hydrogel hybrid through
electrophoresis in the presence of ionic detergents Labeling and imaging of your whole tissue