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The X-CLARITY™ Polymerization Initiator, also known as VA-044, is a thermal free radical initiator. VA-044 is relatively stable at room temperature but, when heated in an aqueous solution, undergoes rapid hemolytic decomposition, releasing cationic free radicals that initiate the polymerization of hydrogel monomers. The X-CLARITY™ systems and reagents standardize, simplify, and accelerate each step of the tissue-clearing process. With the CLARITY method, preserved tissues are embedded in a hydrogel matrix and lipids are actively extracted through electrophoresis to create a stable and optically transparent tissue-hydrogel hybrid that is chemically accessible for multiple rounds of antibody labeling and imaging. Native cytoarchitecture remains intact and even endogenous fluorescence proteins are preserved for robust fluorescence imaging downstream.
