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His-tags are one of the most commonly used tags for protein expression analysis. Conventional metal ion chelators, such as iminodiacetic acid (IDA) and nitrilotriacetic acid (NTA), bind His-tags with low affinities in the range of 10 µM. The biotechrabbit™ Tris-NTA complexes three NTA groups that bind a 6XHis-tag with an affinity four orders of magnitude higher (1 nM) than possible with conventional chelators (10 µM). The binding of His-tags is stoichiometric, and single-molecule detection is possible. Binding is reversible: bound His-tags can be released with imidazole or ethylenediaminetetraacetic acid (EDTA).
Two biotechrabbit™ Tris-NTA versions are available: a version with a free amine group or a version that is conjugated to biotin. It can be used in many applications, including protein detection and labeling, coupling proteins, lipids, or cells to surfaces, protein purification, and reversible protein modification.
NOTICE TO PURCHASER: LIMITED LICENSE
The use of this product for research purposes is covered by a license from QIAGEN Group. No rights to use this product to perform or offer diagnostic, commercial testing, companion diagnostic, or other commercial services for money or money's worth are granted by the supply of this product expressly, by implication, or by estoppel.
Should you wish to use this product for any other purpose not covered by this license, please contact QIAGEN Corporate Business Development at bd@qiagen.com.
