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Strep-Tactin® Superflow® high-capacity columns are ready-to-use for the purification of strep-tagged proteins <90 kDa via affinity chromatography. These gravity flow columns contain highly cross-linked agarose coated with streptavidin variant Strep-Tactin®, which captures proteins fused to a Strep-tag®II or a Twin-Strep-tag®. The straightforward purification produces a highly pure (>95%) protein preparation. After regeneration, the columns can be reused multiple times.
It should be noted that biotinylated proteins also bind to Strep-Tactin® Superflow® high capacity. If biotinylated proteins are expected, BioLock should be used. Furthermore, if large (sample or buffer) volumes have to be applied, we recommend the use of the WET FRED system (IB 2-0911-001).
