Learn how to use this product
Strep-Tactin® Superflow® high capacity is a resin that can be used for the purification of strep-tagged proteins <90 kDa via affinity chromatography. The product is optimized for column-based use and is suitable for HPLC/FPLC. It consists of highly cross-linked agarose beads coupled to the streptavidin variant Strep-Tactin®. Strep-Tactin® binds to proteins fused to a Strep-tag®II or a Twin-Strep-tag®, enabling a straightforward purification of tagged proteins from mixed samples, reaching a purity of >95%. Due to the targeted elution, tedious titration steps are not necessary. In addition, regeneration allows for the reuse of the resin.
Compared to the Strep-Tactin® Superflow (IB 2-1206-025), the high-capacity version has a higher density of Strep-Tactin®, making it suitable for highly expressed proteins. This resin is also an alternative for proteins that are difficult to elute from our Strep-Tactin®XT 4Flow® resins.
Please note that biotinylated proteins also bind to Strep-Tactin® Superflow® high capacity. If biotinylated proteins are expected, BioLock should be used. Furthermore, as this resin is a 50% suspension, 2 ml of resin is required for packing a 1 ml gravity column.
