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StarGateĀ® Entry Vector is for fast protein expression cloning and is designed to insert the PCR product of a certain gene of interest (GOI) to generate a Donor Vector. StarGate cloning via the pENTRY-IBA vector provides a tool for the systematic and rapid screening of the best working expression conditions for a certain GOI, where nothing is known from the literature. After initial pENTRY cloning, the GOI in the resulting Donor Vector can be easily transferred by a simple one-tube reaction into a multitude of expression vectors (Acceptor Vectors) providing different features.
