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pDSG-IBA102 is a small transient expression vector encoding a C-terminal Twin-Strep-tag® that is specially developed for use in combination with the MEXi mammalian expression system. The vector carries an ampicillin resistance cassette for the selection of transformed E. coli cells and the ColE1 replication origin (pUC) for a high plasmid copy number. In addition, it contains the human cytomegalovirus (CMV) immediate-early promoter for high-level expression and the origin of replication from Epstein-Barr Virus (oriP) for extrachromosomal replication driven by EBNA-1 expressed by MEXi-293E cells.
The BM40 secretory signal peptide transfers the expressed protein into the medium. During translocation from the cytosol, the signal peptide is removed from the protein by endogenous proteases. It should be noted that cloning into pDSG-IBA acceptor vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. Besides the direct cloning of the gene of interest into pDSG-IBA vectors with Esp3I, another option via an Entry Vector is possible.
