Learn how to use this product
The RTS Wheat Germ LinTempGen Set, His6-tag, can rapidly generate linear expression constructs ready for use with RTS Wheat Germ kits. PCR-generated linear templates allow fast protein expression from wild-type or modified DNA sequences. The PCR-based template generation can be used to add epitope-tag sequences for antibody detection, introduce mutations, change codon usage, and generate truncated proteins.
Linear templates for protein expression can be used for initial, rapid screening for expression and to avoid the time-consuming step of cloning into expression plasmids. Gene-specific primers can be used for RTS Wheat Germ and E. coli LinTempGen sets, enabling easy transfer of products from any expression system to both the RTS Wheat Germ and E. coli platforms. The linear templates can be cloned into the RTS pIVEX Wheat Germ vector and used with RTS 100/500 Wheat Germ CECF kits for expression scale-up.
