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The RTS Linear Template Kit Plus expedites the generation of multiple templates for parallel protein expression. The constructs are well suited for expression in insect cell-free lysates and E. coli. Due to compatible restriction sites in the adapter primers, products can be easily cloned into pIX3.0 vectors for large-scale in vitro expression.
The kit contains primers providing regulatory elements required for optimal transcription and translation in cell-free expression systems. Specially designed 5'-untranslated regions reduce the formation of secondary structure in the translation initiation region, one of the most common causes of low expression rates. A Strep-tag II or His-tag can be added to either terminus, significantly simplifying protein purification and detection.
A signal sequence enables the expression of functional proteins containing disulfide-bonds or membrane proteins in RTS 100 Insect Membrane or Disulfide kits. A signal peptide can be introduced using the N-terminal primer containing a melittin sequence. This enables protein translocation into microsomal vesicles derived from insect cell endoplasmic reticulum.
