Learn how to use this product
The RTS Linear Template Fab Kit is designed for rapid PCR-based generation of templates directly used in the E. coli Fab and Insect kits. The kit enables functional screening of antibody fragments and disulfide proteins and facilitates the assignment of biological functions to genes. The templates can be generated with optional affinity tags and/ or signal peptide sequences for protein purification and translocation into microsomes.
The RTS Linear Template Fab Kit generates templates with elements to drive efficient coupled in vitro transcription and translation reactions for the synthesis of proteins with intra- and intermolecular disulfide bonds. The kit is a well-suited product for functional screening of disulfide proteins and the optimization of constructs. One kit contains sufficient primer solutions to perform 96 standard protein expression reactions with volumes of 25 μl.
