Learn how to use this product
The RTS 100 E. coli LinTempGen Set is designed to rapidly generate linear expression constructs ready for use with RTS 100 E. coli HY, Fab, and insect kits. PCR-generated linear templates allow fast protein expression from wild-type or modified DNA sequences. The PCR-based template generation can be used for applications such as adding epitope-tag sequences for antibody detection, introducing mutations, changing codon usage, and generating truncated proteins.
Linear DNA templates for protein expression can be used for initial, rapid screening for expression and avoid the time-consuming step of cloning into expression plasmids. Gene-specific primers can be used for RTS Wheat Germ and E. coli LinTempGen sets, enabling easy transfer of products from any expression system to both the RTS Wheat Germ and E. coli platforms. The linear templates can be cloned into RTS pIVEX E. coli vectors and used with RTS 100/500/9000 E. coli CECF kits for expression scale-up.
