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RevertAid Reverse Transcriptases possess an RNA-dependent and DNA-dependent polymerase activity and an RNase H activity specific to RNA in RNA-DNA hybrids. The enzyme is a recombinant version of the wildtype M-MuLV RT and differs by structure, catalytic properties, and optimal activity temperature. It is capable of efficiently synthesizing full-length first-strand cDNA up to 13 kb. You can use RevertAid Reverse Transcriptases for a wide variety of applications:
First-strand cDNA synthesis for RT-PCR and real-time RT-PCR Synthesis of cDNA for cloning and expression Generation of labeled cDNA probes for microarrays DNA labeling Analysis of RNA by primer extension
If you don't need your enzyme to have RNase H activity to obtain high yields of full-length cDNA from long templates, you can choose the RevertAid H Minus Reverse Transcriptase variant. Complete kits, which include the RevertAid Reverse Transcriptase, TH K1621, and TH K1622, are available for easy use.
