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pDSG-IBA103 is a small transient expression vector encoding a C-terminal Twin-Strep-tag® and was specially developed for use with the MEXi mammalian expression system. The vector carries an ampicillin resistance cassette for the selection of transformed E. coli cells and the ColE1 replication origin (pUC) for a high plasmid copy number. In addition, it contains the human cytomegalovirus (CMV) immediate-early promoter for high-level expression and the origin of replication from Epstein-Barr Virus (oriP) for extrachromosomal replication driven by EBNA-1 expressed by MEXi-293E cells.
It should be noted that cloning into pDSG-IBA acceptor vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pDSG-IBA vectors with Esp3I, another option via a so-called entry vector is possible.
