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innuPREP Plasmid Mini Kit 2.0 (Spin Filter with lid) - 500 reactions
Innuscreen
SKU
IN 845-KS-5041500
€ 635,00 excl tax
Learn more about this product
The process is based on the combination of an alkaline lysis and binding of the plasmid DNA to a filter membrane after precipitation of the chromosomal DNA and bacterial proteins. Bound plasmid DNA is then washed and finally eluted by using a low-molar buffer. The isolated plasmid DNA can be used for a number of sensitive downstream applications, like transfection, cloning, PCR or sequencing. The new procedure also allows for the processing of 0.5 ml to 15 ml of bacterial cultures using a tabletop centrifuge (after lysis). With the new filter membrane, up to 80 µg pDNA can be isolated from one sample.
Specifications of innuPREP Plasmid Mini Kit 2.0
Average yield - Depending on the sample and culture volume
Typical yield from 2 ml (high copy Plasmid): approx. 6 – 20 µg
Typical yield from 15 ml (high copy Plasmid): approx. 60 – 80 µg
Starting material Bacteria cell cultures(0.5 to 15 ml) or pellets (gram+ & gram-) & yeast
Extraction of high copy plasmid DNA/ low copy plasmid DNA, P1 constructs and cosmid DNA
Binding capacity: Approx. 80 µg pDNA
Extract: DNA
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