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biotechrabbit™ Heat Labile Uracil–DNA Glycosylase selectively degrades uracil-containing PCR products. After performing PCR or RT-PCR using dUTP instead of dTTP, PCR products remain intact after treatment with Heat Labile Uracil–DNA Glycosylase. In contrast, contaminating DNA (i.e., not amplified) is degraded. Heat Labile Uracil–DNA Glycosylase is entirely and irreversibly inactivated by moderate heat treatment at 50⁰C, allowing contamination control in RT-qPCR. The enzyme hydrolyses the N-glycosylic bond between the deoxyribose sugar and the base in uracil-containing DNA, leaving an abasic (apyrimidinic) site in DNA, but the enzyme does not modify uracils in RNA.
Heat Labile Uracil–DNA Glycosylase is highly active at temperatures between 20–40°C. No cofactors or divalent cations are required for the activity, and the enzyme is active in most PCR and RT-PCR buffers. Although the enzyme is active at a pH between 6.5 and 9.0, the optimal pH 7.5 is in 50 mM NaCl.
