| Type | HBSS |
| Phenol Red | No |
| Volume | 500 ml |
Hanks' Balanced Salt Solution (HBSS) with Ca and Mg, without Phenol Red, is a buffered salt solution. It can be used in various cell culture applications, such as rinsing cells, transporting cells or tissue samples, and diluting cells for counting.
The essential function of a balanced salt solution is to maintain pH and osmotic balance and provide cells with water and essential inorganic ions. When using HBSS as a buffer, it can neutralize small amounts of acid or base and act as a stabilizer to maintain the physiological pH level of a solution. The formulations of HBSS containing calcium and magnesium are generally used as transport media or for reagent preparation.