| Region of origin | South America |
| Serum Modification | Heat Inactivated |
| Volume | 500 ml |
Fetal Bovine Serum Advanced (FBS Advanced), Heat Inactivated, is used for in vitro cultivation of numerous cell types and has reduced batch-to-batch variations compared to standard FBS. FBS Heat inactivated can be used for immunological studies and for culturing sensitive cells. FBS improves various cell culture applications through its high levels of embryonic growth-promoting factors, which are essential for successful cell culturing. Further, FBS contains various small molecules, such as amino acids, sugars, lipids, and hormones.
Serum is the blood component obtained after coagulation and removal of cellular components. Besides serum proteins, it contains growth factors, amino acids, and hormones. This comprehensive mix makes serum one of the most essential supplements for supporting cell growth and proliferation for in vitro cell culture. Of particular interest is FBS. It is especially rich in growth factors and low in antibodies, which may influence the cell culture work.
Raw fetal bovine serum is a natural byproduct of the meat and dairy industry. It is obtained from bovine blood showing significant batch variations due to various environmental influences, e.g., nutrition or health state of animals. Batch-to-batch variations of fetal bovine serum can majorly impact the reproducibility of scientific results. Capricorn Scientific's™ sophisticated treatment process during pooling and filtering significantly reduces batch-to-batch variation in FBS Advanced. Furthermore, the serum content is reduced by adding high-quality albumin to achieve stable performance. Usually, once successfully tested on a cell line, no further batch testing is necessary.
Heat inactivation leads to the inactivation of the complement system, antibodies, and other active enzymes. It has to be done in a carefully controlled process to avoid damaging cell growth-promoting properties of the serum and to reduce the formation of unwanted precipitates. The process involves heating the serum in a shaking water bath at precisely +56 °C for 30 minutes. The shaking helps to avoid the formation of proteins and other forms of precipitates. After 30 minutes, the serum is cooled back down to room temperature as quickly as possible to avoid excessive exposure to heat, which can damage components such as growth factors and vitamins.
This product is collected in South America.