| Volume | 500 ml |
| Phenol Red | Yes |
| Glutamine | With L-Glutamine |
| Type |
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The DMEM/F-12 formulation is a 1:1 mixture of Dulbecco's Modified Eagle Medium (DMEM) and Ham's F-12 and a widely used basal medium for supporting the growth of many different cell types. This formulation of DMEM/F-12 is supplemented with L-glutamine and 15 mM HEPES. It provides the basic nutrients for the growth of a variety of mammalian cell lines, especially when supplemented with FBS. In the past, DMEM was shown to successfully cultivate primary cells, such as fibroblasts, neurons, HUVECs, smooth muscle cells, or even cell lines, such as HEK-293, HeLa, Cos-7, and PC-12.
DMEM is based on Eagle's Minimal Essential Medium (EMEM) but has a fourfold higher concentration of amino acids and vitamins. Ham's F-12, on the other hand, is based on Ham's F-10 medium. A common practice is the supplementation of DMEM with FBS, generally at a concentration of 10%, and this is often necessary because the medium does not contain any growth hormones, lipids, or proteins essential for cell growth. DMEM contains a buffer to maintain pH levels and usually comes with phenol red as a pH indicator. Cells cultivated in DMEM or any other medium containing the bicarbonate buffer system must be kept in a controlled CO2 environment of 5-10%. With the added phenol red, pH changes from 6.2 (yellow) to 8.2 (red) can be monitored.
Each high-quality cell culture media by Capricorn Scientific is manufactured according to the original formula. We offer modifications essential to the consistent performance and stability of the culture medium. This DMEM variant has been tested to support the growth of your cells optimally.