| Protein-free (>10kD) | Yes |
| Phenol Red | Yes |
| Non-Animal Origin (NAO) | Yes |
| Glutamine | Without |
| Chemically defined | Yes |
| Cell type | CHO |
CHO|ONE Feed 1 and Feed 2 are feed supplements specifically matched to the core medium CHO|ONE E. Combining these products increases fed-batch productivity with Chinese Hamster Ovary (CHO) cells 3- to 5-fold compared to batch culture conditions. CHO|ONE Feed 1 contains concentrated amino acids, salts, vitamins, trace elements, and a carbon source.
Further, CHO|ONE Feed 1 comes without Insulin & without L-Glutamine and is supplemented with Pluronicâ„¢. CHO|ONE Feed 2 is a balanced formulation of concentrated amino acids. For use of CHO|ONE Feed 1 and 2, you can refer to proposed protocols to facilitate easy and efficient identification of a feeding strategy. The innovative CHO|ONE Media Systemâ„¢ was invented for small- and large-scale protein production and can be used as a growth medium for CHO-K1, CHO-S, and CHO-DG44 cell lines.
The CHO|ONE Media Systemâ„¢ was designed in close cooperation with a biopharmaceutical cooperation partner and is highly suitable for fed-batch cultures. It improves the yield and concentration of recombinant proteins by optimizing the selection of essential nutrients and growth-promoting factors.