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biotechrabbit™ CAPITAL 1-Step qRT-PCR Probe Master Mix provides outstanding performance for real-time PCR quantification of RNA templates, including mRNA, viral RNA, and total RNA from a broad range of targets. The master mix ensures high sensitivity and specificity in single and multiplex detection, making it a well-suited choice for extremely low-copy-number targets in pathogen detection.
CAPITAL 1-Step qRT-PCR Probe Master Mix uses reverse transcriptase technology and buffer chemistry for efficient cDNA synthesis and QPCR in a single tube. To enable the use of the kit on qPCR platforms with different reference dye concentration requirements, three formats are available. A kit containing no ROX and versions containing low (LRox) or high (HRox) concentrations of ROX.
Info: The recommended annealing temperature is 2°C above primer Tm (use gradient PCR to optimize the annealing temperature).
