Learn how to use this product
biotechrabbit™ CAPITAL qPCR Probe Master Mix is well-suited for quantifying genomic, cDNA, and viral sequences and performs well in single and multiplex qPCR. The high sensitivity provided by the mix is ideal for the detection of low-abundance DNA targets in various applications, such as pathogen detection.
CAPITAL qPCR Probe Master Mix combines enzyme and buffer chemistry for efficient extension and early Ct in single and multiplex qPCR. To enable the use of the kit on qPCR platforms with different reference dye concentration requirements, three formats are available. A kit containing no ROX and versions containing low (LRox) or high (HRox) concentrations of ROX.
Info: The recommended annealing temperature is 2°C above primer Tm (use gradient PCR to optimize the annealing temperature).
