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Biotechrabbit™ lyophilized CAPITAL 1-Step qRT-PCR Probe Master Mix is a lyophilized (freeze-dried) version of the liquid equivalent. This stabilized format allows for storage and shipment without cooling.
Specifically optimized for real-time PCR quantification of various RNA templates, including total RNA, mRNA, and viral RNA, the master mix ensures high specificity and sensitivity in single and multiplex detection scenarios. This makes it well-suited for extremely low-copy-number targets in pathogen detection.
The CAPITAL 1-Step qRT-PCR Probe Master Mix incorporates proprietary reverse transcriptase technology and buffer chemistry, enabling efficient cDNA synthesis and quantitative PCR within a single tube.
Info: The recommended annealing temperature is 2°C above primer Tm (use gradient PCR to optimize the annealing temperature).
