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biotechrabbit™ ApStarTaq™ DNA Polymerase is an aptamer-based hot-start enzyme and the first choice for fast PCR reactions up to 3 kb. The biotechrabbit™ ApStarTaq™ DNA Polymerase is of exceptional quality and purity, ensuring the highest performance, and is suitable for standard and fast PCR cycling in both endpoint and real-time assays. It provides high product yields with low background and without primer–dimer formation or non-specific priming.
The aptamer binds to Taq DNA Polymerase and inhibits the enzyme activity at temperatures below 45°C, ensuring full hot-start functionality. Furthermore, there is no need for separate heating or denaturation steps as the enzyme is released during standard PCR cycling conditions, which allows for fast PCR reactions.
Info: The recommended annealing temperature is 2°C above primer Tm (use gradient PCR to optimize the annealing temperature).