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The biotechrabbit™ AllScript Reverse Transcriptase is designed for sensitive and highly specific reverse transcription. It guarantees top performance in standard reverse transcription, rapid amplification of cDNA ends (RACE), synthesis of double-stranded cDNA for cloning, RT-PCR and qRT-PCR, or RNA analysis by primer extension. Its high affinity to RNA allows transcription of complex secondary RNA structures and targets in low copy number, leading to high cDNA yields.
AllScript Reverse Transcriptase is a multifunctional enzyme including RNA-dependent and ssDNA-dependent DNA polymerase and RNase H activity. The RNase H activity is specific to RNA hybridized to cDNA, with no effect on the pure RNA template, resulting in improved performance of subsequent PCR.